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KMID : 0357319960310020145
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Journal of the Korean Society for Microbiology
1996 Volume.31 No. 2 p.145 ~ p.154
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Detection of elt Gene Encoding Heat-labile Toxin of Escherichia coli by Polymerase Chain Reaction
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이상운
정석찬/박용호/우희종
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Abstract
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This study has been performed to establish specific polymerase chain reaction (PCR) primers for the detection of elt gene encoding heat-labile toxin (LT) of enterotoxigenic Escherichia coli. Further, the study has also shown that the established
PCR
assay can be applied to detect LT-producing E. coli strains isolated from pigs with diarrhea using elt gene-specific primers.
@ES The obtained results were as follows.
@EN 1. PCR assay using elt gene-specific primers was established and this differenciates LT-producing E. coli from non-LT-producing E. coli and other strains.
2. Developed PCR assay using elt gene-specific primers can detect up to 1 pg of purified template DNA in agarose gel analysis.
3. Using developed PCR assay, 4 LT-producing strains among 29 isolates from pigs with diarrhea were able to be detected. This result is completely correspondant to that of Chinese hamster ovary cell assay.
The results have indicated that PCR assay using elt gene-specific primers developed in this study can be a rapid and sensitive method to detect LT-producing strains among ETEC and resident E. coli.
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KEYWORD
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